Peptides: BPC-157 Protocol — Reference Card

This is a reference card for BPC-157 protocols. The critical caveat upfront: every protocol number on this page is extrapolated from animal studies. No human dose-finding trials have been completed. The doses, frequencies, and cycle lengths below represent the community-used approximations derived from rat study data, not clinically validated human protocols.

Dosing Protocol Reference Table

Route	Dose	Frequency	Cycle Length	Target Application	Notes
Subcutaneous (preferred)	200–400mcg	1–2x per day	4–6 weeks	General healing (tendon, gut, systemic)	Inject near injury site when possible; abdominal fat otherwise
Intramuscular	200–400mcg	Once daily	4–6 weeks	Muscle or tendon injury	Inject into or near the affected muscle; rotate sites
Oral (experimental)	250–500mcg	Once daily	4–6 weeks	Gut healing (based on rat data only)	No human BA data; significant uncertainty; potentially useful for GI-specific conditions
Nasal	Not established	Not recommended	—	—	Insufficient data; not a validated route for BPC-157

Cycle structure: 4–6 weeks on, minimum 4–6 weeks off. BPC-157 is not a hormonal compound and does not suppress the HPG axis. No post-cycle therapy (PCT) is required or indicated.

Reconstitution Steps

Step	Action	Notes
1. Verify vial	Check label: peptide name, quantity (e.g., 2mg or 5mg), expiry	Confirm you have lyophilized (freeze-dried) powder — should appear as white cake or powder
2. Gather supplies	Bacteriostatic water, 3mL syringe + 23g needle for reconstitution, insulin syringes for dosing	Do NOT use sterile water — benzyl alcohol in bacteriostatic water prevents bacterial growth
3. Clean vial septa	Wipe top of both vials with 70% isopropyl alcohol swab	Let dry before inserting needle
4. Add water	Draw 2mL bacteriostatic water; inject slowly into BPC-157 vial at angle against glass wall	Inject slowly; avoid vigorous agitation which can degrade peptide
5. Swirl gently	Swirl (do not shake) until powder dissolves completely	Vigorous shaking creates foam and can degrade peptide structure
6. Record and label	Write reconstitution date and volume on vial	Critical for accurate dosing; 2mL in 2mg vial = 1000mcg/mL
7. Store	Refrigerate at 2–8°C; use within 4 weeks	Do not freeze reconstituted peptide; protect from light

Dose Calculation Example

Standard reconstitution: 2mg BPC-157 + 2mL bacteriostatic water = 1000mcg/mL

Target Dose	Volume to Draw	Insulin Syringe Units (100U syringe)
200mcg	0.20mL	20 units
250mcg	0.25mL	25 units
300mcg	0.30mL	30 units
400mcg	0.40mL	40 units

If using a 5mg vial with 2mL water: concentration is 2500mcg/mL. Divide target dose by 2500 to get mL.

Legal Status (Abbreviated)

Jurisdiction	Status	Notes
USA	Research chemical; not scheduled	FDA 503B compounding banned 2023; see full detail at bpc157-fda-status
UK	Not scheduled	No licensed product
Australia	Schedule 4	Prescription required; ASADA prohibited
Canada	Gray market	No DIN; no Health Canada approval
EU	Generally unscheduled	No EMA-approved product

For full FDA timeline and WADA status, see the BPC-157 FDA Status page.

Injection Technique Notes

For subcutaneous injection near an injury site:

• Clean the skin with an alcohol swab; let dry
• Pinch a fold of skin near the target area (1-2 inches from injury)
• Insert insulin syringe at 45° angle for subcutaneous delivery
• Inject slowly; withdraw needle; apply gentle pressure if needed
• Do not rub the injection site — this can disperse the peptide away from the target area

Rotate injection sites across sessions to prevent localized tissue irritation. Use a new insulin syringe for every injection — reuse dramatically increases infection risk.